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uc berkeley macrolab vector 2 bt  (Addgene inc)


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    Structured Review

    Addgene inc uc berkeley macrolab vector 2 bt
    Uc Berkeley Macrolab Vector 2 Bt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/uc+berkeley+macrolab+vector+2+bt/pET+His6+TEV+LIC+cloning+vector+(2B-T)+(Plasmid+%2329666)/pm41093992-237-29-34
    Average 94 stars, based on 88 article reviews
    uc berkeley macrolab vector 2 bt - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Construct:

    Article Title: Bacterial Shedu immune nucleases share a common enzymatic core regulated by diverse sensor domains.
    Article Snippet: .. Shedu-DNL (residues 171-380 of B. cereus Shedu) constructs were cloned into UC Berkeley Macrolab vector 2-BT (Addgene #29666), which encodes an N-terminal TEV protease-cleavable His6 tag. .. All proteins except wild type Shedu-DNL were recombinantly expressed in E. coli strain Rosetta2 pLysS (EMD Millipore) in 2x YT medium.

    Article Title: Identification of the bacteriophage nucleus protein interaction network
    Article Snippet: .. Full-length phage gp2 protein sequences were codon-optimized and synthesized (Invitrogen/GeneArt), then cloned into UC Berkeley Macrolab vector 2-BT (Addgene, catalog number 29666) to generate constructs with N-terminal TEV protease-cleavable His 6 -tags. .. Proteins were expressed in E. coli strain Rosetta 2 (DE3) pLysS (EMD Millipore).

    Article Title: Bacterial Shedu immune nucleases share a common enzymatic core regulated by diverse sensor domains
    Article Snippet: .. Shedu-ΔNL constructs were cloned into UC Berkeley Macrolab vector 2-BT (Addgene #29666), which encodes an N-terminal TEV protease-cleavable His 6 tag. .. All proteins except wild type Shedu-ΔNL were recombinantly expressed in E. coli strain Rosetta2 pLysS (EMD Millipore) in 2x YT medium.

    Article Title: Identification of the bacteriophage nucleus protein interaction network.
    Article Snippet: .. Full-length phage gp2 protein sequences were codon-optimized and synthesized (Invitrogen/GeneArt), then cloned into UC Berkeley Macrolab vector 2-BT (Addgene, catalog number 29666) to generate constructs with N-terminal TEV protease-cleavable His6-tags. .. Proteins were expressed in E. coli strain Rosetta 2 (DE3) pLysS (EMD Millipore).

    Clone Assay:

    Article Title: Bacterial Shedu immune nucleases share a common enzymatic core regulated by diverse sensor domains.
    Article Snippet: .. Shedu-DNL (residues 171-380 of B. cereus Shedu) constructs were cloned into UC Berkeley Macrolab vector 2-BT (Addgene #29666), which encodes an N-terminal TEV protease-cleavable His6 tag. .. All proteins except wild type Shedu-DNL were recombinantly expressed in E. coli strain Rosetta2 pLysS (EMD Millipore) in 2x YT medium.

    Article Title: Identification of the bacteriophage nucleus protein interaction network
    Article Snippet: .. Full-length phage gp2 protein sequences were codon-optimized and synthesized (Invitrogen/GeneArt), then cloned into UC Berkeley Macrolab vector 2-BT (Addgene, catalog number 29666) to generate constructs with N-terminal TEV protease-cleavable His 6 -tags. .. Proteins were expressed in E. coli strain Rosetta 2 (DE3) pLysS (EMD Millipore).

    Article Title: Bacterial Shedu immune nucleases share a common enzymatic core regulated by diverse sensor domains
    Article Snippet: .. Shedu-ΔNL constructs were cloned into UC Berkeley Macrolab vector 2-BT (Addgene #29666), which encodes an N-terminal TEV protease-cleavable His 6 tag. .. All proteins except wild type Shedu-ΔNL were recombinantly expressed in E. coli strain Rosetta2 pLysS (EMD Millipore) in 2x YT medium.

    Article Title: Identification of the bacteriophage nucleus protein interaction network.
    Article Snippet: .. Full-length phage gp2 protein sequences were codon-optimized and synthesized (Invitrogen/GeneArt), then cloned into UC Berkeley Macrolab vector 2-BT (Addgene, catalog number 29666) to generate constructs with N-terminal TEV protease-cleavable His6-tags. .. Proteins were expressed in E. coli strain Rosetta 2 (DE3) pLysS (EMD Millipore).

    Article Title: Short 5’ UTRs serve as a marker for viral mRNA translation inhibition by the IFIT2-IFIT3 antiviral complex
    Article Snippet: .. For expression of the IFIT2-IFIT3 complex, we cloned codon-optimized genes encoding M. musculus Ifit2 and Ifit3 into separate plasmid vectors for expression in E. coli , with Ifit2 cloned into UC Berkeley Macrolab vector 2-BT (Addgene #29666; ampicillin resistant) encoding a TEV protease-cleavable His6-tag, and Ifit3 cloned into UC Berkeley Macrolab vector 13S-A (Addgene #48323; spectinomycin resistant) with no tag. .. Plasmids were co-transformed into E. coli Rosetta pLysS cells (EMD Millipore) and grown overnight at 37°C in LB plus carbenicillin and spectinomycin.

    Article Title: The IFIT2-IFIT3 antiviral complex targets short 5' untranslated regions on viral mRNAs for translation inhibition.
    Article Snippet: .. For expression of the IFIT2–IFIT3 complex, we cloned codon-optimized genes encoding M. musculus Ifit2 and Ifit3 into separate plasmid vectors for expression in E. coli, with Ifit2 cloned into UC Berkeley Macrolab vector 2-BT (Addgene, 29666; ampicillin resistant) encoding a TEV protease-cleavable His6-tag, and Ifit3 cloned into UC Berkeley Macrolab vector 13S-A (Addgene, 48323; spectinomycin resistant) with no tag. .. Plasmids were co-transformed into E. coli Rosetta pLysS cells (EMD Millipore) and grown overnight at 37 °C in LB plus carbenicillin and spectinomycin.

    Synthesized:

    Article Title: Identification of the bacteriophage nucleus protein interaction network
    Article Snippet: .. Full-length phage gp2 protein sequences were codon-optimized and synthesized (Invitrogen/GeneArt), then cloned into UC Berkeley Macrolab vector 2-BT (Addgene, catalog number 29666) to generate constructs with N-terminal TEV protease-cleavable His 6 -tags. .. Proteins were expressed in E. coli strain Rosetta 2 (DE3) pLysS (EMD Millipore).

    Article Title: Identification of the bacteriophage nucleus protein interaction network.
    Article Snippet: .. Full-length phage gp2 protein sequences were codon-optimized and synthesized (Invitrogen/GeneArt), then cloned into UC Berkeley Macrolab vector 2-BT (Addgene, catalog number 29666) to generate constructs with N-terminal TEV protease-cleavable His6-tags. .. Proteins were expressed in E. coli strain Rosetta 2 (DE3) pLysS (EMD Millipore).

    Plasmid Preparation:

    Article Title: Bacterial Shedu immune nucleases share a common enzymatic core regulated by diverse sensor domains
    Article Snippet: .. Shedu-ΔNL constructs were cloned into UC Berkeley Macrolab vector 2-BT (Addgene #29666), which encodes an N-terminal TEV protease-cleavable His 6 tag. .. All proteins except wild type Shedu-ΔNL were recombinantly expressed in E. coli strain Rosetta2 pLysS (EMD Millipore) in 2x YT medium.

    Article Title: Short 5’ UTRs serve as a marker for viral mRNA translation inhibition by the IFIT2-IFIT3 antiviral complex
    Article Snippet: .. For expression of the IFIT2-IFIT3 complex, we cloned codon-optimized genes encoding M. musculus Ifit2 and Ifit3 into separate plasmid vectors for expression in E. coli , with Ifit2 cloned into UC Berkeley Macrolab vector 2-BT (Addgene #29666; ampicillin resistant) encoding a TEV protease-cleavable His6-tag, and Ifit3 cloned into UC Berkeley Macrolab vector 13S-A (Addgene #48323; spectinomycin resistant) with no tag. .. Plasmids were co-transformed into E. coli Rosetta pLysS cells (EMD Millipore) and grown overnight at 37°C in LB plus carbenicillin and spectinomycin.

    Article Title: Loop extrusion-mediated plasmid DNA cleavage by the bacterial SMC Wadjet complex
    Article Snippet: UC Berkeley Macrolab vector 2-AT , Addgene , 29665. .. UC Berkeley Macrolab vector 2-BT , Addgene , 29666. .. UC Berkeley Macrolab vector 13S-A , Addgene , 48323.

    Article Title: The IFIT2-IFIT3 antiviral complex targets short 5' untranslated regions on viral mRNAs for translation inhibition.
    Article Snippet: .. For expression of the IFIT2–IFIT3 complex, we cloned codon-optimized genes encoding M. musculus Ifit2 and Ifit3 into separate plasmid vectors for expression in E. coli, with Ifit2 cloned into UC Berkeley Macrolab vector 2-BT (Addgene, 29666; ampicillin resistant) encoding a TEV protease-cleavable His6-tag, and Ifit3 cloned into UC Berkeley Macrolab vector 13S-A (Addgene, 48323; spectinomycin resistant) with no tag. .. Plasmids were co-transformed into E. coli Rosetta pLysS cells (EMD Millipore) and grown overnight at 37 °C in LB plus carbenicillin and spectinomycin.

    Expressing:

    Article Title: Short 5’ UTRs serve as a marker for viral mRNA translation inhibition by the IFIT2-IFIT3 antiviral complex
    Article Snippet: .. For expression of the IFIT2-IFIT3 complex, we cloned codon-optimized genes encoding M. musculus Ifit2 and Ifit3 into separate plasmid vectors for expression in E. coli , with Ifit2 cloned into UC Berkeley Macrolab vector 2-BT (Addgene #29666; ampicillin resistant) encoding a TEV protease-cleavable His6-tag, and Ifit3 cloned into UC Berkeley Macrolab vector 13S-A (Addgene #48323; spectinomycin resistant) with no tag. .. Plasmids were co-transformed into E. coli Rosetta pLysS cells (EMD Millipore) and grown overnight at 37°C in LB plus carbenicillin and spectinomycin.

    Article Title: The IFIT2-IFIT3 antiviral complex targets short 5' untranslated regions on viral mRNAs for translation inhibition.
    Article Snippet: .. For expression of the IFIT2–IFIT3 complex, we cloned codon-optimized genes encoding M. musculus Ifit2 and Ifit3 into separate plasmid vectors for expression in E. coli, with Ifit2 cloned into UC Berkeley Macrolab vector 2-BT (Addgene, 29666; ampicillin resistant) encoding a TEV protease-cleavable His6-tag, and Ifit3 cloned into UC Berkeley Macrolab vector 13S-A (Addgene, 48323; spectinomycin resistant) with no tag. .. Plasmids were co-transformed into E. coli Rosetta pLysS cells (EMD Millipore) and grown overnight at 37 °C in LB plus carbenicillin and spectinomycin.



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